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Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine embryos

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

《农业科学与工程前沿（英文）》 2019年第6卷第1期页码 66-72 doi: 10.15302/J-FASE-2018229

摘要：

Porcine viral diarrhea is an acute and highly contagious enteric disease of pigs that causes huge economic losses worldwide. Porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are the main pathogens responsible for piglet viral diarrhea. However, currently there is no specific drug available for the effective treatment of viral diarrhea. Therefore, it is necessary to seek an effective method to diminish PEDV and TGEV infection rates. RNA interference has been applied successfully to inhibit the virus replication. It provides a potential strategy for breeding resistant pigs. In this study, four promoters and four short hairpin RNA (shRNA) vectors with LoxP sites at each end of the selectable marker genes were constructed to target PEDV and TGEV. These vectors were then transfected into porcine fetal fibroblasts, G418 resistant transfectants were confirmed by PCR and transgenic SCNT porcine blastocysts were obtained. These results have paved the way for future production of marker-free transgenic resistant to PEDV and TEGV pigs by SCNT.

关键词： piglet diarrhea RNAi PEDV TGEV transgenic resistance breeding

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Observations on pathological and histochemical changes in piglet livers infected with Taenia saginata

LINGHU Yan, ZHU Wujun, BAO Huai′en, CHEN Yan

《医学前沿（英文）》 2007年第1卷第3期页码 258-263 doi: 10.1007/s11684-007-0049-8

摘要： To study the pathological and histochemical characteristics of lesions in piglet livers infected with () throughout the different stages, piglets were fed with eggs of and raised in isolation in an animal center to establish the infection model with normal piglets as control. The pathological changes in the piglet livers were observed after the infection using liver sections stained with hematoxylin and eosin. Histochemical methods were used to check the changes in lipid, glycogen and protein content in the liver. The data collected by image analysis were analyzed statistically with Statistical Package for the Social Science. The results show that -exposed piglets were indeed infected. Inflammatory reactions began on the fourth day and progressed rapidly. Kupffer cell hyperplasia, hepatic hydropic degeneration and ballooning degeneration were found in the 10th 20th days after infection. Hepatic central veins and hepatic sinusoids were dilated and congested. Spotty necrosis occurred in some local liver tissues. In the 40th–60th days, granulomatous reactions and mild hepatocirrhosis were the main lesions. In the 70th–80th days, hepatocirrhosis and bile duct proliferation were observed in the liver. In the different stages, lipid drops were increased while glycogen and protein levels were decreased to some degree. There was a significant difference in metabolism between the infected group and the control group (〈0.01). It is concluded that pigs are the favorable intermediate host of and its infection can result in serious pathological and histochemical lesions in host organs.

关键词： hydropic degeneration significant difference -exposed favorable intermediate different

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infections among diarrheal outpatients in Shanghai: a retrospective case study

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《医学前沿（英文）》 2018年第12卷第1期页码 98-103 doi: 10.1007/s11684-018-0614-3

摘要：

Cyclospora cayetanensis is a foodborne and waterborne pathogen that causes endemic and epidemic human diarrhea worldwide. A few epidemiological studies regarding C. cayetanensis infections in China have been conducted. During 2013, a total of 291 stool specimens were collected from patients with diarrhea at a hospital in urban Shanghai. C. cayetanensis was not detected in any of the stool specimens by traditional microscopy, whereas five stool specimens (1.72%, 5/291) were positive by PCR. These positive cases confirmed by molecular technology were all in the adult group (mean age 27.8 years; 2.94%, 5/170) with watery diarrhea. Marked infection occurred in the rainy season of May and July. Sequence and phylogenetic analyses of the partial 18S rRNA genes of C. cayetanensis isolated showed intra-species diversity of this parasite. This study showed, for the first time, that C. cayetanensis is a pathogen in outpatients with diarrhea in Shanghai, albeit at a low level. However, the transmission dynamics of this parasite in these patients remain uncertain.

关键词： Cyclospora cayetanensis outpatients with diarrhea stool specimens 18S rRNA gene

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Preliminary results of gentamycin combined with sodium bicarbonate for prevention of irinotecan-induced diarrhea

Qi MEI MM, Zhe CAO MM, Hua XIONG MD, Yuan CHEN MD,

《医学前沿（英文）》 2009年第3卷第4期页码 470-474 doi: 10.1007/s11684-009-0077-7

摘要： The aim of this paper was to observe the clinical effect of gentamycin combined with sodium bicarbonate for the prevention of irinotecan-induced diarrhea. A total of 98 patients with stage IV cancers were recruited and divided into a prevention group (52 patients) and a control group (46 patients). All patients received the chemotherapy including irinotecan. The prevention group received gentamycin and sodium bicarbonate before the use of irinotecan for 4 days; the control group did not receive any prevention. The use of gentamycin and sodium bicarbonate resulted in significantly higher stool pH ( < 0.001), while the incidence of diarrhea by irinotecan was reduced (prevention group 13.70% control group 34.83%; < 0.001). Gentamycin combined with sodium bicarbonate appears to be useful in preventing the diarrhea induced by irinotecan and reducing the dosage of loperamide and fluid replacement.

关键词： irinotecan diarrhea gentamycin sodium bicarbonate prevention